Gehring, A.G., Albin, D.M., Bhunia, A.K., Kim, H., Reed, S.A., and Tu, S-I. Amass, S., A. K. Bhunia, A. Chaturvedi, D. Dolk, S. Peeta and M. Atallah 2006. Chem. Moreira, H. Kim, and A.K. Two isolates (F1 from food and CHL1250 from patient) had unique ribotype patterns that were not previously reported in the RiboPrinterO database. (C) The real-time fluorescence curves for different concentrations of S. aureus genomic DNA. https://www.frontiersin.org/articles/10.3389/fmicb.2014.00770 Bhunia, A.K. Burkholder, K.M., and Bhunia, A.K. P-044, p497. doi: 10.1016/j.bios.2015.09.058, Zhang, M., Wang, X., Han, L., Niu, S., Shi, C., and Ma, C. (2018). Liu, A.K. Detection of low levels of pathogenic Listeria monocytogenes in 14 - 20 h using immunoseparation and cytotoxicity techniques. WebThe conventional methods used to detect foodborne pathogen are time consuming and laborious. Proceedings of the Society for PhotoOptical Instrumentation Engineers. 1.Introduction. 1 . Published Therefore, in this work, we firstly utilized RNA one-step detection of SEA method to realize the detection of viable foodborne pathogen S. aureus. J. Biophoton 4:236-243. Nanotechnol. A laser light scattering system has also been developed to distinguish bacterial colonies grown on solid agar plates. WebThe pre-PCR processing step, including sample preparation and nucleic acid extraction, is a critical step in foodborne virus detection. Sensors 6:796-807. Chem. Institute of Food Technologist General Meeting, Chicago, IL. Characterization of Staphylococcus aureus strains associated with food poisoning in Shenzhen, China. The advent of biotechnology has introduced new technologies that led to the emergence of rapid diagnostic methods and altered food testing practices. Genome sequencing-based typing methods include multiple-locus sequence typing (MLST), clustered regularly interspaced short palindromic repeats (CRISPR), and whole genome sequencing (WGS). Leibniz Institut fr Analytische Wissenschaften, ISAS Campus, Dortmund, Germany. 2002. Atlanta, GA. June 5-9, 2005, Abstr. Proceeding of SPIE 5996:599603-1- 10. Editors: Yan, X., Juneja, V., Huang, L. DEStech Publications, Inc. Lancaster, PA. pp 599-624. In the United States, nearly half a million hospitalizations and 50,000 deaths occur resulting from S. aureus each year (Schlecht et al., 2015). Briefly, one pair of specific primers bound with the targeted DNA fragments by invading the denaturing bubbles and induced DNA polymerase to extend the chain. Control and prevention of Listeria monocytogenes in the dairy industry. Bhunia. The Korean Society for Microbiology and Biotechnology. Burkholder, K.M., and Bhunia, A.K. Yang, L., P.P. Aberdeen, Scotland. Kim, and A.K. International Association of Food Protection annual meeting, Columbus, OH. For example, S. aureus is the main cause of nosocomial infections and community-acquired diseases, including deep-seated, endocarditis, abscesses and bacteria, which lead to toxic and septic shock syndromes (Abdalhai et al., 2014). With improved antibody and recognition molecules, PMB coupled with fiber-optic sensor was able to detect very low levels (102 cfu) of L. monocytogenes. Real-Time, on-plate rapid detection and identification of Bacillus cereus-group using light scattering sensor. A two-step detection system specific for pathogenic L. monocytogenes has also been developed. Status: Food safety is a significant public health issue in both developed and developing countries. FOIA Bhunia. Performance evaluation of a multiplex selective enrichment broth, SEL by proteomic analysis and immunoassay. Mishra, K., Kim, H., Mendonca, M., Aroonnual, A., and Bhunia, A.K. Morgan, D. Ess, B.-K. Hahm, A. Kothapalli, A. Valadez, T. Geng, A.K. Chem. In addition, we are also developing sensor platforms for simultaneous detection of multiple pathogens. 2000. Jagadeesan, B., La, D., Kihara, D., and Bhunia, A.K. NON-TECHNICAL SUMMARY: With the increase in reports of foodborne infections, great attention has been given to the development of new/improved methods for rapid detecting microbial pathogens. Characterization of surface proteins of Cronobacter muytjensii using monoclonal antibodies and MALDI-TOF Mass spectrometry. 2003. Zhao, J., S. Jedlicka, J. Lannu, A.K. Ohk, S-H., and Bhunia, A.K. PMC Hahm, B.-K., Y. Maldonado, A.K. A laser light scattering system was used to demonstrate its ability to distinguish bacterial colonies grown on solid agar plates. A rapid procedure for isolating chromosomal DNA from Lactobacillus species and other Gram-positive bacteria. Naschansky, K.M. Burkholder, J.A. Kim, K.P., B.K. Adhesion characteristics of Listeria adhesion protein (LAP)expressing Escherichia coli to Caco-2 cells and of recombinant LAP to eukaryotic receptor Hsp60 as examined in a surface plasmon resonance sensor. 2006. 003A-12. Liposome-doped nanocomposites as artificial-cell-based biosensors: Detection of listeriolysin O. American Society for Microbiology General Meeting, Washington, DC. and Bhunia, A.K. Bae, E., Banada, P.P., Huff, K., Bhunia, A.K., Robinson, J.P., and Hirleman, E.D. Bhunia, A.K., Nanduri, V., Bae, E. and Hirleman, E.D., 2010. Study of Listeria monocytogenes survival / inactivation on a new conveyor design. 2002. 2013 May 18-22, 2003. Boston, MA. Development and optimization of two-dimensional centering algorithm for bacterial rapid detection system using forward scattering. Influence of secreted Listeria Adhesion Protein (LAP) on transepithelial translocation and cytokine release during Listeria infection in Caco-2 cell model. Look for the .htaccess file in the list of files. This media is currently being evaluated for detection of Listeria monocytogenes, Escherichia coli O157:H7 and Salmonella enterica from various artificially inoculated food samples using fiber optic-based immunosensors. Hahm, B.K., Kim, H., and Bhunia, A.K. A pair of S. aureus specific primers were designed for the SEA reaction by targeting hypervariable V2 region of 16S rDNA and the amplification reaction was finished about 1 h. The results of amplification reaction could be observed by the naked eyes with a significant color change from light yellow to red to realize the colorimetric detection of S. aureus. In addition, detection of multipathogens using a single sensor platform is of major importance because it can provide safety assessment of a product very rapidly and can save money for pathogen testing. Banerjee, P., P. P. Banada, J.L. Bhunia, A.K., Banada, P.P., Banerjee, P., Valadez, A., and Hirleman, E.D. Proceedings of SPIE. 2010. 2023 Feb 9;13(2):246. doi : 10.3390 elongated assessment time and the need for skilled personnel are some of the shortcomings of the existing conventional methods. Lactobacillus paracasei Expressing LAP Reduces Cell Invasion, Translocation and Cytotoxicity of L. monocytogenes in Caco-2 Cells. Food Technol. Anaerobic environment increases surface localization of Listeria Adhesion Protein (LAP) and promotes infectivity of Listeria monocytogenes. Institute of Food Technologist Annual Meeting. MeSH Nelson, R.K. Singh, A.K. Feb 5-7, 2002. Microbiol. Tu, and A.K. Rapid detection, characterization, and enumeration of foodborne pathogens. Examination of cytopathic effect and apoptosis in Listeria monocytogenes-infected hybridoma B-lymphocyte (Ped-2E9) line in vitro. The site is secure. Samples were divided into 25 g each with a sterile knife. The rapid and precise monitoring and detection of foodborne pathogens are some of the most effective ways to control and prevent human foodborne infections. In this example the file must be in public_html/example/Example/. The use of non-invasive sampling techniques for live animals especially came into focus with the 1990s outbreak of bovine spongiform encephalopathy that was linked to the human outbreak of Creutzfeldt Jakob's Disease. American Society for Microbiology General Meeting, New Orleans, LA. Utilization of optical forward scatter image biological database: food-borne pathogen colony differentiation and detection. J. Nutr. Unauthorized use of these marks is strictly prohibited. Bhunia, and M.R. 6381:638107-1-638107-8. Finally, 62C was chosen as the optimal reaction temperature (Figure S1) in this work. 2004. Enzyme-Linked Immunosorbent Assay (ELISA): is considered a valid rapid and easy alternative to conventional methods able to detect this pathogen in 2007. Wampler, A.K. Journal of Biological Engineering 2:6. J. Bio-physical modeling of forward scattering from bacterial colonies using scalar diffraction theory. Bae, E., N. Bai, Bhunia, A.K., Robinson, J.P. and Hirleman, E.D. Modern methods are based on molecular biology techniques like PCR, RFLP, DNA microarray assay, immunological techniques like ELISA, biophysical and WebWe demonstrated its application on two widespread foodborne bacteria, namely, E. coli O157:H7 and Streptococcus aureus, using specific crRNA targeting rfbE and nuc gene, respectively. Bhunia. SecA2 aids in secretion of proteins involved in both pathogenesis and house-keeping function in pathogenic and nonpathogenic bacteria.113th General Meeting of American Society for Microbiology, May 18-21, 2013, Denver, CO. Abst # 1341. There are general standard methods for determination of the foodborne pathogens and mycotoxins in food products including conventional methods such as culture and microscopic methods, chemical and biological methods (immunological, molecular genetic methods, gel diffusion), there are also more rapid methods which Infect. Rapid Sample Processing for Foodborne Pathogen Detection via Crossflow Microfiltration. May 19-23, 2002. 16(3):337-342. J. Infect. Abstr. 2013 Therefore, it is necessary to develop a simple and rapid detection method for S. aureus. Bhunia, J.P. Robinson, and M.R. In addition, various genomic typing methods (amplified fragment length polymorphism (AFLP) and repetitive PCR (Rep-PCR) methods were used to generate genomic fingerprint patterns for E. coli O157:H7 strains. 2013 Mar;97(5):1829-40. doi: 10.1007/s00253-013-4692-5. Huff, K. 2008. Gut Pathog. Microbiol. Fiber optic - based immunosensor was able to detect E. coli O157:H7 at an initial contamination level of 1 cfu/g of ground beef after 4-6 h and Listeria monocytogenes with a sensitivity of 100,000 cells/ml in meat samples after 20 h enrichment employing the automated RATOR system. P-094, p498. Mira Miralles M, Maestre-Carballa L, Lluesma-Gomez M, Martinez-Garcia M. Foods. In addition, we also developed methods to control pathogens using antimicrobial coated nanoparticles on products and probiotics in mammalian host. Foodborne pathogens are a serious threat to global food safety. We have isolated several enterohemorrhagic E. coli (EHEC) strains from naturally contaminated ground beef (an ongoing project from Alabama A&M University). Olga Shevchuk. Hirleman, and A.K. Furthermore, L. monocytogenes-specific biorecognition molecules also provided novel strategies for its control using probiotics or antimicrobial peptide coated nanoparticles.Publications, Progress 10/01/09 to 09/30/10OutputsOUTPUTS: Improvement in sensor design and performance evaluations for pathogens and toxin continue to be the focus of our group. Horizon Scientific Press, Norfolk, UK. Unable to load your collection due to an error, Unable to load your delegates due to an error. 1, The Science of Homeland Security, Editors: Purdue University Press, West Lafayette, IN. 2006. PLoS One.6 (6):e20694. New Orleans, LA. P-070, p494. Gene 489:76-85. Just click. June 1-5, 2008, P-074. Citation: Rickus, K. Ragheb, C. Corvalan, J. P. Robinson, and A.K. 2011. Serology is still an important tool in preventing foodborne pathogens to enter the human food supply through meat and milk from animals. However, the sensitivity of these methods are low and their operations are usually very difficult and tedious, which make them hard to popularize (Kimura et al., 2013). Burkholder, K.M., Kim, K-P., Mishra, K., Medina, S., Hahm, B-K., Kim, H., and Bhunia, A.K. Chapter 25; pp 414-421. Accessibility Are you using WordPress? 098-31, pp. Prevalence of antibodies reactive to pathogenic and nonpathogenic bacteria in preimmune serum of New Zealand white rabbits. WebThese more rapid detection and identification methods will result in quicker trackback and recall, potentially leading to reduction in exposure to contaminated food and therefore number of illnesses. 137, 1380413806. Epidemiology of foodborne diseases: a worldwide review. Detection of S. aureus by colorimetry. Stat. 20 (5): 2058-2060. # End WordPress. Disclaimer. Institute of Food Technologist Annual Meeting, June 24-28, 2006, Orlando, FL Abstr. Status: We also found that the occurrence of the fluorescence signal was significantly delayed with DNA or RNA elimination, which indicated that detecting RNA and DNA simultaneously with the SEA method showed higher sensitivity than that of DNA or RNA independently. Bhunia. Kim, H., M.A.R. CS revised and approved the final version. Proceeding of SPIE. Bhunia. Status: Subsequently fibers were exposed to L. monocytogenes-specific monoclonal antibody (C11E9)-conjugated to a fluorescene dye (Cy5) and signals were obtained from a laser photodiode detector (Analyte 2000). 2009. Antibody immobilization on waveguides using a flow through system show improved Listeria monocytogenes detection in an automated fiber optic biosensor: RAPTOR. 6080:155-162. The whole SEA detection procedure for real samples took only 12 h, which was so time-saving compared with the traditional plating method taking as long as 72 h. Thus, the SEA method provided a simple, rapid and equipment-free detection platform. Abstr. Microchimica Acta 175, 105112. WebStaphylococcus aureus (S. aureus) contamination in food safety has become a worldwide health problem. P-031, p494-495. 32, 5256. 2012. S. aureus, Staphylococcus aureus; SEA, strand exchange amplification; POCT, point-of-care testing; PCR, polymerase chain reaction; ELISA, enzyme linked immunosorbent assay; NASBA, nucleic acid sequence-based amplification; LAMP, loop-mediated isothermal amplification; SDS, sodium dodecyl sulfate; HPLC, high performance liquid chromatography; NTC, no target control; PAGE, polyacrylamide gel electrophoresis. Early detection would reduce ware-house holding time for products, and prevent potential foodborne Listeria monocytogenes related outbreaks and mortality.Publications, Progress 10/01/02 to 09/30/03OutputsWe are continuing our efforts in developing biosensor tools for the detection of Listeria monocytogenes. Put the custom structure back if you had one. 2009. Either way, please contact your web host immediately. Five other bacterial fluids, including L. monocytogenes, S. typhimurium, S. castellani, V. parahemolyticus, and E. coli were used to verify the specificity and anti-interference of the SEA method (Yang et al., 2016). Open J. Appl. 058-19., pp 91. 2009. The 2-cys peroxiredoxin deficient Listeria monocytogenes displays impaired growth and survival in the presence of hydrogen peroxide in vitro but not in mouse organs. 2013. Likewise, 11 outbreaks of Staphylococcal food poisoning were reported between 2006 and 2009 in Shenzhen, China, which ranked the second most frequent cause of bacterial food poisoning (Yan et al., 2012). Biosensors, Foodborne Pathogen Detection. M-028, p396. 2013. WebA portable multi-channel turbidity system by real-time loop-mediated isothermal amplification (LAMP) method for rapid detection of pathogens allows a rapid, small size, low cost, SEA detection kits were obtained from Qingdao Navid Biotechnology Co. Ltd. (China). Bookshelf Bae, E. and Bhunia, A.K. Los Angeles, CA. In Advances in Food and Nutrition Research. The target for colorimetric assay, specificity and anti-interference experiments. Jaradat, K. Naschansky, M. Shroyer, M. Morgan, R. Gomez, R. Bashir, and M. Ladisch. Adhesion, Invasion, and translocation characteristics of Listeria monocytogenes serotypes in a Caco-2 cell and mouse model. Analysis of environmental Escherichia coli isolates for virulence genes using the TaqMan system. 2006. Hahm, B.K., T. Geng, and A.K. Abstr. June 1-5, 2008, P-095. doi: 10.1128/AEM.01165-12, Yang, X., Zhang, J., Yu, S., Wu, Q., Guo, W., Huang, J., et al. 2008. Applied and Environmental Microbiology 69 (6): 3640-3645. 144. All strains contained virulence genes, inlA, inlB, actA, hlyA, plcA and plcB with expected product size in PCR assay except for the actA gene. EHEC isolates were also examined for genomic fingerprint profiles using randomly amplified polymorphic DNA fragmentation (RAPD) assay. Wereley. 33:166-171. Bio-physical modeling of time-resolved forward scattering by Listeria colonies. Purdue University. Optical biosensors in foodborne pathogen detection. This has been the main strength of rapid methods such as real-time Polymerase Chain Reaction (PCR). FoodMicro2008. About 13% of beef samples were found to be positive for E. coli O157:H7 strains. This study was financially supported by grants from the National Natural Science Foundation of China (21475071, 31670868, 21675094), the Taishan Scholar Program of Shandong (ts201511027), and the Shandong Province Natural Science Fund Major Basic Research Project (ZR2017ZC0123). System automation for bacterial colony detection and identification instrument via forward-scattering. 127 p. Lathrop, A.L., P.P. Previous detection methods struggle to meet the current demands. be positive for E. coli O157:H7. Goodridge, L.D., Fratamico, P., Christensen, L.S., Hoorfar, J., Griffiths, M., Carter, M., Bhunia, A.K., O Kennedy, R. 2011. Year Published: 2005. The sensitivity of the SEA detection method for S. aureus was evaluated with different dilutions of genomic DNA and DNA fragments. A .gov website belongs to an official government organization in the United States. Rapid pathogen screening tools for food safety. Considering the wide existence in nature and severe effects of S. aureus, we have to make surveillance on it in various foods to ensure our health. Kim, and A.K. 67:185-239, Salm, E., D. Marchwiany, D., Liu, Y.S., Morisette, D., He, Y., Bhunia, A., and Bashir, R. 2011. Epub 2013 Jan 18. Bhunia. 2009. Microbes Infect. Editor: S. Taylor, Vol. Nature Nanotechnology. PhD Thesis. Nakatsu. 1999. The results showed that SEA could be used to detect the concentration of S. aureus genomic DNA as low as 400 pg/L (Figure 2C). Ability of sensors to detect and identify multiple pathogens and toxins on a single sensor platform is essential to provide total microbiological quality of products and to reduce cost for product testing. 2013. F1-1356/229. American Society for Microbiology General Meeting. When you get a 404 error be sure to check the URL that you are attempting to use in your browser.This tells the server what resource it should attempt to request. Sept 17-20, 2011, Chicago. Detection of foodborne pathogens at an early stage is very important to control food quality and improve medical response. Careers. 2000. The culture fluids of S. aureus with L. monocytogenes, S. typhimurium, V. parahemolyticus, S. castellani, and E. coli were used to demonstrate the specificity and anti-jamming capacity of the SEA method. Mialon, M., Tang, Y., Singh, A.K., Bae, E., and Bhunia, A.K. American Society for Microbiology General Meeting. 2012. A Listeria adhesion protein-deficient Listeria monocytogenes strain shows reduced adhesion primarily to intestinal cell lines. A portable cell based optical detection device for rapid detection of Listeria and Bacillus toxins. May 23-27, 2004, Abstr. 27 (3): 179-188. Efficacy of this sensor to detect L. monocytogenes from other meat products is currently under investigation.Publications, Progress 10/01/01 to 09/30/02OutputsDeveloping a sensitive detection tool for viable and virulent Listeria monocytogenes is the major focus of this project. B.-K. Hahm, J. Fiser, S. Yeary, and A.K. 65(2):38-43. Gen. Meet. New Orleans, LA. Electrical detection of dsDNA and polymerase chain reaction amplification. Bae, E. and A. K. Bhunia. Detection of pathogenic Escherichia coli in food and environmental samples using PCR. Compatible antibodies as a pair of capture and detection reagents were selected by epitope mapping and the optimal concentration of capturing antibody and detecting antibody were determined by cross titration. Abstract # 152-18. SPIE Proceedings. A., Hnsch, G. M., Filler, S. G., et al. 2010 May 30;139 Suppl 1:S79-94. Two-step methods were developed: First, concentration of Listeria cells from food and second specific detection by biosensor-based probes such as (a) interdigitated microsensor electrode (IME)-chip, (b) enzyme-fluorescence assay to measure Listeria interaction with animal cells, and (c) antibody-coupled fiber optic biosensor. May 21-25, 2006, Abstr. Crit Rev Food Sci Nutr. Epub 2017 Jul 11. Rapid detection of foodborne pathogens with high sensitivity and specificity is becoming an urgent requirement in health safety, medical diagnostics, environmental safety, and controlling food quality. Electrical characterization of microorganisms using microfabricated devices. 2006. 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Testing practices meet the current demands it is necessary to develop a simple and rapid and! System specific for pathogenic L. monocytogenes in Caco-2 cell and mouse model h using immunoseparation and cytotoxicity techniques coli:!